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HEMATOXYLIN & EOSIN AND PAPANICOLAOU – SEND & CHOOSE

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Hematoxylin - Eosin (H&E) staining is the oldest and most used stain in Anatomic Pathology laboratories, it is of essential importance for diagnosis even today. For this reason, Diapath S.p.A. offers a range of staining, developed according to the reference literature and optimized with the aim of constant product improvement, allowing to take advantage of excellent results, enhancing nuclear details and emphasizing intensity gradations of cytoplasmic stains.

Papanicolaou staining is a polychrome staining for cytological specimens (both gynecological and non-gynecological). The method is based on an aqueous-based nuclear staining and two alcohol-based cytoplasmic staining. Hematoxylin is normally used for blue-purple staining of nuclei, OG6 and EA50 as cytoplasmic dyes. OG6 is the staining solution that highlights cytoplasm in cells and keratinized areas.EA50 is a polychrome solution that enables specific differentiation of cytoplasm of squamous epithelial cells.

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de Diapath vous permet de choisir la combinaison de différents types d'hématoxyline et d'éosine qui convient le mieux à vos besoins et à ceux de votre laboratoire, en 4 étapes simples.

1. COMPLETE :
fill in the form and choose the H&E PAP combination that you want to try

2. SEND:
prepare your slides in a box and wait for the courier.
* For cytology samples (smears or monolayer spots) fix them with fixative spray before sending them

3. TESTING AT DIAPATH HEADQUARTER:
your slides stained with reliable Diapath H&E PAP staining solution

4. CHOOSE:
once received your stained slides, analyze them and choose the best H&E PAP combo for your lab. needs

The advantages for you:

QUALITY IN DIAGNOSTICS
Different intensity gradations for high quality nuclear and cytoplasmic stains

IMPROVED FORMULATIONS
Optimized solutions aimed at constant improvement

VARIETY OF SOLUTIONS
Possibility to choose combinations that best suit specific needs

READY TO USE
Ready to use solutions

Diapath Hematoxylin
Strong *
Medium *
Delicate *
* indicative scale of values, directly proportional to the concentration of Hematoxylin in gr. The final result is strictly dependent on the type and duration of the color change
Mayer
hematoxylin
Mayer
hematoxylin Ready to use nuclear dye for regressive staining. Sometimes used in progressive ones where nuclear counterstaining is required to emphasize the cytoplasmic component. Short staining time and differentiation with spring water.
Staining time Histology: 5’ – 10’ Packaging C0302: 500mL, C0303:1Lt, C0305:2.5Lt, C0306: 5Lt
Harris for
histology
Harris for
histology Ready to use clear nuclear dye. Used in both regressive and progressive histological staining.
Staining time Histology: 3’ – 7’ Packaging C0282: 500mL, C0283:1Lt, C0285:2.5Lt, C0286: 5Lt
Cytopath Harris hematoxylin
Cytopath Harris hematoxylin Ready to use solution for blue staining of nuclei in association with the cytoplasmic dyes OG6 and EA50.
Staining time Cytology: 1' - 3' Packaging CP502: 500mL, CP503:1Lt, CP506:5Lt
Carazzi
hematoxylin
Carazzi
hematoxylin Ready to use solution for regressive staining. Like Mayer's hematoxylin, it can be used as a progressive nuclear counterstain. Short staining time, followed by differentiation with spring water.
Staining time Histology: 5’ – 10’ Packaging C0203: :1Lt, C0206: 5Lt
Gill I hematoxylin
Gill I hematoxylin Characterized by a modest concentration of hematoxylin. Ready to use solution used as progressive staining. The color of the nuclei takes on a typically purplish shade.
Staining time Cytology: 1' - 5' Packaging CP813:1Lt, CP815:2.5Lt, CP816:5Lt
Gill II hematoxylin
Gill II hematoxylin Characterized by an average concentration of hematoxylin. Ready to use solution for histology and cytology.
Staining time Histology: 3’ – 5’ Cytology: 1' - 3' Packaging CP813:1Lt, CP815:2.5Lt, CP816: 5Lt
Gill III hematoxylin
Gill III hematoxylin Characterized by a high concentration of hematoxylin. Ready to use solution that allows to obtain intense nuclear colors in a short time. Particularly suitable for intraoperative staining.
Staining time Histology: 1' - 3' Cytology: 2' - 3' Packaging CP823:1Lt, CP825:2.5Lt, CP826: 5Lt
DIAPATH EOSIN AND PAP EA50 – OG6
Strong *
Medium *
Delicate *
* indicative scale of values, strictly dependent on post-eosin dehydration steps
Eosin G or Y 0.5% alcoholic
Eosin G or Y 0.5% alcoholic Ready to use cytoplasmic dye with a weakly acidic alcohol base which allows to distinguish, by means of a characteristic differentiation of shades from red to light pink
Staining time Histology: 3’ – 5’ Packaging C0352: 500mL, C0353:1Lt, C0355:2.5Lt, C0356: 5Lt
Eosin G or Y polychromatic 1% aqueous
Eosin G or Y polychromatic 1% aqueous Ready to use cytoplasmic dye with aqueous base which allows to distinguish, by means of a characteristic differentiation of shades from red to light pink
Staining time Histology: 2’ – 5’ Packaging C0362: 500mL, C0363:1Lt, C0365:2.5Lt, C0366: 5Lt
Eosin-Floxin
Eosin-Floxin Ready to use cytoplasmic dye slightly acidic. Made up of a mixture of eosin and floxin powder in alcoholic solution. It allows to obtain a more intense cytoplasmic staining than eosin.
Staining time Histology: 1’ – 5’ Packaging C0343:1Lt, C0345:5Lt
EA50
EA50 Ready to use cytoplasmatic dye with alcholic base which allows to distinguish by means of characteristics differentiation of shades from pink to blue.
Staining time Cytology: 2:30' Packaging CP602:500ml, CP603:1Lt, CP605:2,5Lt, CP606:5Lt
OG6
OG6 Ready to use cytoplasmatic dye with alcholic base which allows to distinguish by means of keratinized cells or areas in orange.
Staining time Cytology: 00:40'' Packaging CP702:500ml, CP703:1Lt, CP705:2,5Lt, CP706:5Lt
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1 Company data 2 Combinations selection 3 Parcel collection
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TOTAL
Legend AMayer hematoxylin BHarris for histology CCytopath Harris hematoxylin DCarazzi hematoxylin EGill I hematoxylin FGill II hematoxylin GGill III hematoxylin
1Eosin G or Y 0.5% alcoholic 2Eosin G or Y polychromatic 1% aqueous 3Eosin-Floxin 4OG6-EA50
ATTENTION: The slides delivered must not report any sensitive data attributable to a subject, they must report only and exclusively the indication of the type of tissue and the thickness of the section (eg infiltrating carcinoma of the breast - 3µm). A maximum of 5 slides can be sent per case. The stained slides will be used exclusively for your evaluation of the result of the different hematoxylin-eosin combination PAP chosen as per the table above and will not have any diagnostic purpose. It is required that a stained glass slide be sent for each combination chosen. If the slides are not correlated with the already stained ones, the request will not be accepted.
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The company DIAPATH S.p.A. to use the images of the colors obtained with the slides sent, for commercial and marketing purposes, in paper, electronic, web form or on social platforms and without territorial limitations, renouncing from now on any claim, recourse or inhibitory action.

The company DIAPATH S.p.A. to use the images of the colors obtained with the slides sent, for commercial and marketing purposes, in paper, electronic, web form or on social platforms and without territorial limitations, renouncing from now on any claim, recourse or inhibitory action.

The company DIAPATH S.p.A. to the processing of data for any marketing / promotion of the Controller's products, activities aimed at analyzing consumer habits and choices, activities relating to the development of studies and market research, sending information and / or material relating to its products as per the information pursuant to Article 13 of EU Regulation no. 679/2016 of 27 April 2016.

Toutes les informations et données contenues ici, doivent être considérées comme privées et confidentielles. La diffusion et la publication de ces données et informations à des tiers sont donc interdites. Vous devez garder confidentielles toutes les informations et données portées à votre connaissance lors de la consultation et les conserver avec des mesures de sécurité et un degré de soin non inférieurs à ceux accordés à vos propres informations confidentielles. L'accès à cet espace nécessite l'acceptation pleine et entière des conditions énumérées ; Diapath se réserve donc le droit de poursuivre tous les contrevenants dans le cadre de la réglementation en vigueur et de demander réparation de tous les préjudices subis.

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